UPR Biology

José E. García-Arrarás


Ph.D., Harvard University, 1981.

Phone: (787)764-0000 ext.2596

E-mail: jegarcia@upracd.upr.clu.edu

Fax: (787) 764-3875

Field of Interest: Development of the Peripheral Nervous System; Neuropeptides and Neurotransmiters. Regeneration of the Nervous System.

Studies to understand the development of neuropeptide and neurotransmitter containing cells from embryogenesis to adulthood. The appearance and development of neuroactive substances in the peripheral nervous system of avian species is studied using radioimmunoanalysis and immunocytochemistry. In vitro systems of embryonic ganglia and of their precursors are used to study the various factors that affect the expression of the neuroactive substances. Invertebrate models are used to study the regeneration of the nervous system. Holothurians, or sea cucumbers, are used to study nerve cell and fiber regeneration. Molecular studies using PCR and cDNA libraries are used to characterize the genes involved in regeneration mechanisms.

Latest Research Projects:

Major Equipment and Facilities Used: Radioisotope room: gamma counter, refrigerated centrifuge, explosion proof hood. Cell culture room: incubators, sterile flow hood, dissection microscope. Immunocytochemistry: fluorescence microscope, microtome, cryostat. Monoclonal antibody facility. HPLC electrochemical detector.

Selected Publications:

Medina-Ortiz, W. and J.E. García-Arrarás. Differential regulation of NPY mRNA expression in embryonic sympathetic and chromoffin cultures by NGF. Dev. Brain Res. In Press.

Méndez, A. T., J. L. Roig-López, P. Santiago, C. Santiago J. E. García-Arrarás. Identification of Hox gene sequences in the sea cucumber Holothuria glaberrima Selenka (Holothuria:Echinodermata). Marine Biotech. In Press.

Ramírez-Ordoñez, R., J. Barreto-Estrada and J. E. García-Arrarás.(1999) Growth effects on the expression of morphological and biochemical properties of avian embrionic sympathetic cells. Emphasis on NGF. Dev. Brain Res. 114:27-36

Finotto, S., K. Krieglstein, A.Schober, F. Deimling, K. Linder, B. Bruhl, K. Beier, J. Metz, J. E. García-Arrarás, J. L. Roig-López, P., Monoghan, W. Schmid, T. Cole, C. Kellendonk, F. Troche, G. Schutz, and K. Unsikcer. (1999) Analysis of mice carring targeted mutations of the glucocorticoid receptor gene argues against an essential role of glucocorticoid signalling for generating adrenal chromaffin cells. Development 126:2935-2944.

García-Arrarás, J.E., L. Díaz-Miranda, I. I. Torres, S. File, L. B. Jiménez, K. Rivera-Bermúdez, E. Arroyo and W. Cruz. (1999) Regeneration of the enteric nervous system in the sea cucumber, Holothuria glaberrima. J. Comp. Neurol. 406:461-475.

García-Arrarás, J.E., and I. Torres-Avillán. (1999) (Development expression of galanin-like immunoreactivity by members of the avian sympathoadrenal cell lineage). Cell Tissue Res. 295:33-41.

García-Arrarás, J.E., L. Estrada-Rodgers, R. Santiago, I. Torres, L. Díaz-Miranda and I. Torres-Avillán. (1998) Cellular mechanisms of intestine regeneration in the sea cucumber, Holothuria glaberrima Selenka (Holothuroidea:Echinodermata). Journal of Experimental Zoology, 281:288-304.

Deimiling, F., S. Finotto, K. Linder, B. Brnhl, J. L. Roig-López, J. E. García-Arrarás, K. Krieglstein, and . Unsicker. (1998). (Characterization of adrenal chromaffin progenitor cell in mice. in: Catecholamines Bridging Basic Science With Clinical Medicine). Edited by David Golstein Advances in Pharmacology vol. 42

Barreto-Estrada, J., L. Medina-Vera, J. de Jesús-Escobar, and J. E. García-Arrarás.(1997). Development of galanin and enkephalin-like immunoreactivities in the sympathoadrenal lineage of the avian embryo; In vivo and in vitro studies. Dev. Neurosci. 19:328-336.

Schober, A., L. Minichiello, M. Keller, K. Huber, P. G. Layer, J. L. Roig-López, J. E. García- Arrarás, Rüdiger Klein and Klaus Unsicker. (1997) Reduced AChE-activity in adrenal medulla and loss of sympathetic preganglionic neurons in TrkA-, but not TrkB-deficient mice. J. Neurosci. 17:891-903.

Díaz-Miranda, L., C.F. Pardo-Reoyo, R. Martínez and J. E. García-Arrarás. (1996). Galanin-like immunoreactivity in the sea cucumber Holothuria glaberrima. Cell Tissue Res. 286:385-391.

Díaz-Miranda, L. and J.E. García-Arrarás, (1995). Pharmacological action of the heptapeptide GFSKLYFamide in the muscle of the sea cucumber Holothuria glaberrima (Echinodermata). Comp. Biochem. Physiol. 110: 171-176.

Ramírez-Ordoñez, R. and J.E. García-Arrarás, (1995). Peptidergic, catecholaminergic and morphological properties of avian chromaffin cells are modulated distinctively by growth factors. Dev. Brain Res. 87: 160-171.

Maynard-Salgado, G. and J.E. García-Arrarás, (1995). Depolarization effects on the peptidergic phenotypes of chick sympathetic and adrenal. Brain Res. 676: 268-276.

Díaz-Miranda, L., R. Blanco and J.E. García-Arrarás, (1995). Localization of GFSKLYFamide in the sea cucumber Holothuria glaberrima (Echinodermata): a light and electron microscopic study. J. Comp. Neurol. 352: 626-640.

García-Arrarás, J.E., Localization of Peptides: Double labeling immunohistochemistry. In: Handbook of Endocrine Research Treatment. pp. 207-225. Academic Press. Inc. San Diego, CA.

Bennet, M.K., J.E. García-Arrarás, L.A. Elferink, K. Peterson, A.M. Fleming, C.D. Hazuka and R.H. Scheller, (1993). The syntaxin family of vesicular transport receptors. Cell 74: 863-873.

García-Arrarás, J.E. and E. Viruet, (1993). Enteric nerve fibers of holothurians are recognized by an antibody to acetylated tubulin. Neurosci. Lett. 157: 153-157.

Smith, J.E. Vyas, and J.E. García-Arrarás, (1993). Selective modulation of cholinergic properties in cultures of avian embryonic sympathetic neurons. J. Neuroscience Res. 34: 346-356.

García-Arrarás, J.E., A.M. Lugo Chinchilla and I. Chévere-Colón, (1992). The expression of neuropeptide Y immunoreactivity in the avian sympathoadrenal system conforms with two models of co-expression for neurons and chromaffin cells. Development 115:617-627.

Díaz-Miranda, L., D.A. Price, M.J. Greenberg, T.D. Lee and J.E. García-Arrarás, (1992). Characterization of two novel neuropeptides from the sea cucumber Holothuria glaberrima. Biol. Bull. 182: 241-247.