Ph.D., University of Chicago, 1970.
Phone: (787)764-0000, x2009
Fax: (787) 764-3875
Field of Interest: Developmental genetics of Drosophila. Mechanisms of chemical
One of our projects is to evaluate the mutagenic potential of some anticancer compounds
and topoisomerase II inhibitors in somatic and germ cells of Drosophila melanogaster
and to relate these findings to their molecular structure. In addition, the effect of
several antimutagenic compounds in the genotoxicity of anticancer drugs and other
compounds is being evaluated.
Another proyect is the characterization of the alcohol dehydrogenase ADH) gene enzime
system in representative species of the cardini group of Drosophila. Our aim is to
contribute to the understanding of the evolution of this gene and its regulatory
components. The species of this group are distributed in tropical America and the
Caribbean. Studies include: determination of alcohol tolerance and enzyme activity; a
genetic analysis of the electrophoretic patterns observed for ADH in these species abd
their hybrids; ontogeny and tissue-specific exspression.
Major Equipment and Facilities Used: Facilities for the preparation of culture media,
cleaning and sterilization of glassware. Drosophila insectary in laboratory with basic
equipment; incubators; refrigerators; disecting microscopes (Zeiss, Bausch & Lomb);
compound microscopes (American Optical, Zeiss); starch, acrilamide and agarose gel
electrophoresis equipment; Laminar Flow Hood (Class II type B).
- Colón-Parrilla, W.V. and Y. Pérez-Chiesa. 1999. Ethanol Tolerance and Alcohol
Dehydrogenase (ADH;EC184.108.40.206). Activity in species of the cardini Group of Drosophila.
Biochemical Genetics (1999) 37(3/4):95-107.
- Pérez-Chiesa, Y., G. Huertas and S. I. Rodríguez, 1995. Genotoxicity evaluation of
3-nitrobenzothiazolo [3,2-a] aquinolinium chloride (NBQ) in the wing somatic mutation and
recombination test (SMART) of drosophila melanogaster.
- Pérez-Chiesa, Y. and Z. Narváez, 1993. Genotoxic evaluation of the
indeoisoquinoline analogues of fagaronine and nitidine in Drosophila melanogaster.
- Pérez-Chiesa, Y., and A. Rodríguez, 1993. Absence of mutagenicity of
benzo[c]phenantridine alkaloids in somatic cells of Drosophila Melanogaster:
Comparison with 7,12-dimethylbenzo [a]anthracene and chrysene.Mutation.
- Pérez-Chiesa, Y., A. Arroyo, D. Rodríguez, A. Rodríguez, O. Cox and H.R.
Retamozo,1991. Absence of mutagenicity of the antitumor drug
4-nitrobenzothiazolo[3,2-a]quinolilium chloride (NBQ) in the germ cells of Drosophila
melanogaster males. Mutation Res. 264:179-182.
- Pérez- Chiesa, Y., and C. N. Cintrón, and A. Rodríguez, 1987. Effect of fagaronine
on chromosome breaks and loss in Drosophila melanogaster. Mutation Res. 188:
- Pérez-Chiesa, Y., and C. N. Cintrón, 1986. Mutagenic effect of fagaronine in Drosophila
melanogaster. Mutation Res.173:193-196.
- Carrasco, C., Y. Pérez-Chiesa and D. Bruck, 1984. Esterases of Drosophila dunni.
Comparative Biochem. and Physiol. 79B: 375-378